HBStart Green qPCR Premix is a ready-to-use master mix supplied at 2× concentration, consisting of DNA polumerase engineered with a proprietary hot-start mechanism, SYBR Green I dye, dNTP, buffer components, etc. The user only needs to template, provide primers, and water.
The chemically modified DNA polymerase is equipped with a proprietary hot-start mechanism that completely inhibits enzyme activity at room temperature to provides improved specificity. The polymerase is re-activated after a few minutes incubation at 95°C.
Free or single-stranded DNA-bound SYBR Green I has a low level of intrinsic fluorescence; however, SYBR Green I exhibits optimal fluorescence when bound to double-stranded DNA. So it can be used on the quantitative detection of target gene based on the principle that an increase in fluorescence intensity is proportional to the amount of double-stranded PCR product produced.
HANBIO's Product Information of qPCR Premix
HANBIO's qPCR Premix product is stored at -20℃ away from light and used within one year. For frequent use in the short term, the premix can be stored at 4℃ away from light. The sybr premix ex taq product has been tested that storing the product at 4℃ for a week or repeatedly freezing and thawing 10 times, does not affect the use of the effect.
Competitive Features and Benefits of Hanbio's qPCR Premix
Simple Operation: The qPCR Premix from Hanbio reduces the number of steps, shorten sample addition time, and minimize contamination risks.
Specificity：As a reliable vector manufacturing company, Hanbio's qPCR Premix's effectively suppress non-specific amplification and significantly improve amplification efficiency.
Stable and Precise: The qPCR Premix is capable of accurate quantification across a wide range of template concentrations, yielding stable and reliable qPCR results.
qPCR Premix Method of Use
- PCR Reaction Mixture
Note:
- It is recommended to apply DNA template in quantities less than 100 ng. Since the copy number of different types of template genes varies, the optimal amount could be determined by developing a gradient dilution series of tests.
- DNA template should be added in less than 10% the volume of the PCR reaction mixture.
- PCR Protocol
Two-step RT-qPCR is best suited for applications requiring high specificity, while three-step TR-qPCR is suited for applications requiring high amplification efficiency.
Hanbio is a biotechnology company that specializes in the research, development, and production of biological products for scientific, medical, and industrial applications. It's great to know that Hanbio offers virus packaging, plasmid vector construction, and a wide range of molecular biology reagents.
